Multiomics Studies Reveal Pathways that Enable Clonal Expansion and Cancer Pribyl LJ, Kay JE, Corrigan JJ, Kohale I, Owiti NA, Volk L, Norales M, Moise AC, and Engelward BP. DNA methylation damage induces persistent transcriptome dysregulation and interferon activation, culminating in clonal expansion and cancer. In preparation.

Tool Quantifies Differences in DNA Repair Among Individuals Ngo LP, Kaushal S, Chaim IA, Mazzucato P, Ricciardi C, Samson LD, Nagel ZD, Engelward BP. CometChip analysis of human primary lymphocytes enables quantification of inter-individual differences in the kinetics of repair of oxidative DNA damage. Free Radic Biol Med. 2021 Oct;174:89-99.doi: 10.1016/j.freeradbiomed.2021.07.033. Epub 2021 Jul 26.PMID: 34324980 NIEHS Paper of the Month.

Collaborative Work Demonstrates a Better Way to Predict Health Hazards from Alkylating Agents in the Atmosphere. Kelly JM, Ivatt PD, Evans MJ, Kroll JH, Hrdina AIH, Kohale IN, White FM, Engelward BP, Selin NE. Global cancer risk from unregulated polycyclic aromatic hydrocarbons. Geohealth. 2021 Sep 1;5(9):e2021GH000401. doi: 10.1029/2021GH000401. PMID: 34589640. In the MIT News. In Medical News. In Science Daily News.

Study points to a key DNA repair protein as a potential genetic susceptibility factor for NDMA-induced cancer. Kay JE, Corrigan JJ, Armijo AL, Nazari IS, Kohale IN, Torous DK, Avlasevich SL, Croy RG, Wadduwage DN, Carrasco SE, Dertinger SD, White FM, Essigmann JM, Samson LD, Engelward BP. Excision of mutagenic replication-blocking lesions suppresses cancer but promotes cytotoxicity and lethality in nitrosamine-exposed mice. Cell Rep. 2021 Mar 16;34(11):108864. doi: 10.1016/j.celrep.2021.108864. PMID: 33730582. In the MIT News. NIEHS Paper of the Month. In Genetic Engineering and Biotechnology News (GEN).

Learn more about a recent public health study that points to a link between NDMA exposure and childhood cancer. Engelward BP. Implications of an epidemiological study showing an association between in utero and NDMA exposure and childhood cancer. Mol Mutagen. 2021 Jun;62(5):288-292. doi: 10.1002/em.22434. Epub 2021 Jun 2.PMID: 33963777
See also our blog and an NIEHS news article.

This paper provides an overview of techniques developed in the Engelward lab to study DNA damage, repair, and mutagenesis. Owiti NA, Nagel ZD, Engelward BP. Fluorescence sheds light on DNA damage, DNA repair and mutations. Trends Cancer. 2021 Mar;7(3):240-248. doi: 10.1016/j.trecan.2020.10.006. Epub 2020 Nov 14.
PMID: 33203608

Three-Dimensional Cell Model Enhances DNA Damage Testing. SpheroidChip: Patterned agarose microwell compartments harboring HepG2 spheroids are compatible with genotoxicity testing. Chao C, Ngo LP, Engelward BP. ACS Biomater. Sci. Eng., Apr 13;6(4):2427-2439. doi: 10.1021/acsbiomaterials.9b01951. Epub 2020 Mar 2. In the NIEHS Superfund Research Program News.

Improved CometChip offers a better way to screen for chemical safety. Ngo LP, Owiti NA, Swartz C, Winters J, Su Y, Ge J, Xiong A, Han J, Recio L, Samson LD, Engelward BP. Sensitive CometChip assay for screening potentially carcinogenic DNA adducts by trapping DNA repair intermediates. Nucleic Acids Res. 2020 Feb 20;48(3):e13. In the MIT News. In the NIEHS Superfund Research Program News. NIEHS Paper of the Month.

MicroColonyChip: A better way to measure cell survival. Ngo LP, Chan TK, Ge J, Samson LD, and Engelward BP. Microcolony size distribution assay enables high-throughput cell survival quantitation. Cell Reports. 2019 26:1668-1678. In the MIT News and on the MIT Homepage. In the NIEHS Environmental Factor. NIEHS Paper of the Month.

CometChip reveals that Colibactin from E. coli creates DNA interstrand crosslinks. Wilson MR, Jiang Y, Villalta PW, Stornetta A, Boudreau PD, Carrá A, Brennan CA, Chun E, Ngo L, Samson LD, Engelward BP, Garrett WS, Balbo S, Balskus EP. The human gut bacterial genotoxin colibactin alkylates DNA. Science. 2019 Feb 15;363(6428).

Demonstration that asthma causes DNA damage points to DNA repair as a susceptibility factor for asthma. Chan TK, Loh XY, Peh HY, Tan WNF, Tan WSD, Li N, Tay IJJ, Wong WSF, Engelward BP. House dust mite-induced asthma causes oxidative damage and DNA double-strand breaks in the lungs. J. Allergy Clin. Immunol. 2016 138:84-96.e1. In the news and on the MIT Homepage

An emerging role for DNA damaging agents in infectious disease: for S. pneumoniae, the ability to secrete genotoxic chemicals is a pathogenicity factor.
Rai P, Parrish M, Tay IJ, Li N, Ackerman S, He F, Kwang J, Chow VT, Engelward BP. Streptococcus pneumoniae secretes hydrogen peroxide leading to DNA damage and apoptosis in lung cells. Proc. Natl. Acad. Sci. USA. 2015 Jun 30;112(26):E3421-30. In the MIT News

Inflammation-induced cell proliferation acts synergistically with DNA damage to induce mutations.
Kiraly O, Gong G, Olipitz W, Muthupalani S, Engelward BP. Inflammation-induced cell proliferation potentiates DNA damage-induced mutations in vivo. PLoS Genetics. 2015 Feb 3;11(2):e1004901. In the MIT News

Graduate Students Criss-Cross the Globe to Work Together: Collaboration between MIT and Thai Researchers enables Application of New Tools to address Global Problems in Public Health. Ge J, Prasongtanakij S, Wood DK, Weingeist DM, Fessler J, Navasummrit P, Ruchirawat M, and Engelward BP. CometChip: a high-throughput 96-well platform for measuring DNA damage in microarrayed human cells. J Vis Exp. 2014

Spontaneous levels of base excision repair shown to pressure homologous recombination in vivo, Kiraly O, Gong G, Roytman MD, Yamada Y, Samson LD, Engelward BP. DNA glycosylase activity and cell proliferation are key factors in modulating homologous recombination in vivo. Carcinogenesis. 2014 Aug 25. pii: bgu177.

Nanoparticles shown to be genotoxic, Watson C, Ge J, Cohen J, Pyrgiotakis G, Engelward BP, Demokritou P. High-throughput screening platform for engineered nanoparticle-mediated genotoxicity using CometChip technology. ACS Nano. 2014 Mar 25;8(3):2118-33. In the MIT News

Second generation mouse model enables studies of homologous recombination in a broad range of tissue types and reveals accumulation of recombination events in somatic stem cells. Sukup-Jackson MR, Kiraly O, Kay JE, Na L, Rowland EA, Winther KE, Chow DN, Kimoto T, Matsuguchi T, Jonnalagadda VS, Maklakova VI, Singh VR, Wadduwage DN, Rajapakse J, So PT, Collier LS, Engelward BP. Rosa26-GFP direct repeat (RaDR-GFP) mice reveal tissue- and age-dependence of homologous recombination in mammals in vivo. PLoS Genet. 2014 Jun5;10(6):e1004299. In the MIT news

CometChip enables high throughput screening of DNA double strand breaks. Weingeist DM, Ge J, Wood DK, Mutamba JT, Huang Q, Rowland EA, Yaffe MB, Floyd S, and Engelward BP. Single cell microarray enables high throughput evaluation of DNA double strand breaks and DNA repair inhibitors. Cell Cycle. 2013 Mar 15;12(6):907-15.
News and Views by Nickoloff J.A., Assaying DNA double-strand break induction and repair as fast as a speeding comet, Cell Cycle. 2013 12:1335-6.

Imaging approaches for suppression of light-induced DNA damage. Ge J, Wood DK, Weingeist DM, Prasongtanakig S, Navasumrit P, Ruchirawat M, Engelward BP. Standard fluorescent imaging of live cells is highly genotoxic. Cytometry A. 2013 Jun;83(6):552-60. Editorial by Tárnok A., Visualization can be harmful for live cells, Cytometry A., 2013 83:521-2.

MIT study suggests that at low dose-rate, radiation poses little risk to DNA. (also featured by MIT as “Best of 2012”) News of this study was dispersed to over 50 sites worldwide. Olipitz W, Wiktor-Brown D, Shuga J, Pang B, McFaline J, Lonkar P, Thomas A, Mutamba JT, Greenberger JS, Samson LD, Dedon PC, Yanch JC, Engelward BP. Integrated molecular analysis indicates undetectable change in DNA damage in mice after continuous irradiation at ~ 400-fold natural background radiation. Environ Health Perspect. 2012 Aug;120(8):1130-6. In the news and on the MIT Homepage

Novel technology for high throughput analysis of DNA damage
Wood DK, Weingeist DM, Bhatia SN, Engelward BP. Single cell trapping and DNA damage analysis using microwell arrays. Proc Natl Acad Sci U S A. 2010 Jun 1;107(22):10008-13. In the news and on the MIT Homepage

Burden of mutant cells is driven primarily by cell division in the pancreas
Wiktor-Brown DM, Kwon HS, Nam YS, So PT, Engelward BP. Integrated one- and two-photon imaging platform reveals clonal expansion as a major driver of mutation load. Proc Natl Acad Sci U S A. 2008 Jul 29;105(30):10314-9. In the MIT news

Recombination mechanisms come to life with animations See Animations
Top 10 downloaded papers in DNA Repair for 2008-2011; Most highly cited article in 2012
Helleday T, Lo J, van Gent DC, Engelward BP. DNA double-strand break repair: from mechanistic understanding to cancer treatment. DNA Repair (Amst). 2007 Jul 1;6(7):923-35.

Visualization of fluorescent recombinant cells reveals accumulation with age
Wiktor-Brown DM, Hendricks CA, Olipitz W, Engelward BP. Age-dependent accumulation of recombinant cells in the mouse pancreas revealed by in situ fluorescence imaging. Proc Natl Acad Sci U S A. 2006 Aug 8;103(32):11862-7.

Indirect induction of homologous recombination is transmissible
Rugo RE, Almeida KH, Hendricks CA, Jonnalagadda VS, Engelward BP. A single acute exposure to a chemotherapeutic agent induces hyper-recombination in distantly descendant cells and in their neighbors. Oncogene. 2005 Jul 28;24(32):5016-25.

Chemical mechanisms of inflammation-induced sequence rearrangements
Kiziltepe T, Yan A, Dong M, Jonnalagadda VS, Dedon PC, Engelward BP. Delineation of the chemical pathways underlying nitric oxide-induced homologous recombination in mammalian cells. Chem Biol. 2005 Mar;12(3):357-69.

Fluorescence detection of recombination in vivo
Hendricks CA, Almeida, KH, Stitt, MS, Jonnalagadda, VS, Rugo, RE, Kerrison, GF, Engelward BP. Spontaneous mitotic homologous recombination at an enhanced yellow fluorescent protein (EYFP) cDNA direct repeat in transgenic mice. Proc. Natl. Acad. Sci. USA. 2003 May 27;100(11):6325-30.

DNA repair enzymes can promote nitric-oxide-induced recombination
Spek EJ, Vuong LN, Matsuguchi T, Marinus MG, Engelward BP. Nitric oxide-induced homologous recombination in Escherichia coli is promoted by DNA glycosylases. J. Bacteriol. 2002 Jul;184(13): 3501-7.

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Publications

Pribyl LJ, Kay JE, Corrigan JJ, Kohale I, Owiti NA, Volk L, Norales M, Moise AC, Ma D, Levine S, Ragan T, Croy RG, Dertinger S, Wadduwage DN, Carrasco S, Essigmann JM, White F, and Engelward BP. DNA methylation damage induces persistent transcriptome dysregulation and interferon activation, culminating in clonal expansion and cancer. In preparation.

Volk L, Norales M, Corrigan JJ, Pribyl LJ, Karjane C, Michelsen E, Carrasco SE, Dulski E, Bugher NA, Blawas M, Swanson M, Croy RG, Essigmann JM, Plata D, Engelward BP. Age and DNA repair shape the risk of mutations, stress responses, and cancer following NDMA-induced DNA methylation damage. In preparation.

Swartz C, Martin L, Rottinger E, Pinkus E, Stankowski L, Owiti N, Roberts D, Pfuhler S, Engelward BP, Recio L. Reproducibility and transferability of HepaRG CometChip assay. In preparation.

Kay JE, Corrigan JJ, Volk LB, Armijo AL, Nazari IS, Toro’s DK, Aylasevich SL, Croy RG, Wadduwage DN, Dertinger SD, Essigmann JM, Samson LD, Carrasco SE, and Engelward BP. Base excision repair and sex differences dictate susceptibility to NDMA-induced mutations, toxicity, and cancer. In preparation.

Chen M, Corless EI, Engelward BP, Sikes H, Swager TM. Optical detection of interleukin-6 using liquid Janus emulsions using hyperthermophilic affinity proteins. ACS Omega (2024) http://doi.org.ezproxy.canberra.edu.au/10.1021/acsomega.4c03959

Armijo AL, Thongararm P, Fedeles BI, Yau J, Kay JE, Corrigan JJ, Chancharoen M, Chawanthayatham S, Samson LD, Carrasco SE, Engelward BP, Fox JG, Croy RG, Essigmann JM. Molecular origins of mutational spectra produced by the environmental carcinogen N-nitrosodimethylamine and SN1 chemotherapeutic agents. NAR Cancer. 27;5(2) (2023) doi: 10.1093/narcan/zcad015.

Collins A, Møller P, Gajski G, Vodenková S, Abdulwahed A, Anderson D, Bankoglu EE, Bonassi S, Boutet-Robinet E, Brunborg G, Chao C, Cooke MS, Costa C, Costa S, Dhawan A, de Lapuente J, Bo’ CD, Dubus J, Dusinska M, Duthie SJ, Yamani NE, Engelward B, Gaivão I, Giovannelli L, Godschalk R, Guilherme S, Gutzkow KB, Habas K, Hernández A, Herrero O, Isidori M, Jha AN, Knasmüller S, Kooter IM et al. Measuring DNA modifications with the comet assay: a compendium of protocols. Nature Protocols, 18(3):929-989 (2023) doi: 10.1038/s41596-022-00754-y

Moise AC, Kay JE, Engelward BP. Transgenic mice harboring direct repeat substrates reveal key underlying causes of homologous recombination in vivo. DNA Repair (Amst). 120:103419 (2022).

Owiti NA, Corrigan JJ, Pribyl LJ, Kay JE, and Engelward BP. Novel In Vivo CometChip reveals NDMA-induced DNA damage and repair in multiple mouse tissues. Int. J. of Mol. Sci., 23(19):11776 (2022).

Moise A, Kay JE, Engelward BP. Transgenic mice harboring direct repeat substrates reveal key underlying causes of homologous recombination in vivo. DNA Repair, 120:103419 (2022).

Owiti NA, Kaushal S, Martin L, Sly J, Swartz CD, Fowler J, Corrigan JJ, Recio L, Engelward BP. Using the HepaCometChip assay for broad-spectrum DNA damage analysis. Curr Protoc. 2022 Sep;2(9):e563. doi: 10.1002/cpz1.563. PMID: 36165707; PMCID: PMC9522315.

Pradhan D, Ding H, Zhu J, Engelward BP, Levine SS. NExtSEEK: Extending SEEK for active management of interoperable metadata. J Biomol Tech. 2022 Feb 2;33(1):3fc1f5fe.db404124. doi: 10.7171/3fc1f5fe.db404124. PMID: 35836998; PMCID: PMC9258913.

Buick JK, Rowan-Carroll A, Gagné R, Williams A, Chen R, Li HH, Fornace AJ Jr, Chao C, Engelward BP, Frötschl R, Ellinger-Ziegelbauer H, Pettit SD, Aubrecht J, Yauk CL. Integrated genotoxicity testing of three anti-infective drugs using the TGx-DDI transcriptomic biomarker and high-throughput CometChip® assay in TK6 cells. Front. Toxicol. 2022 Sep 23;4:991590. doi: 10.3389/ftox.2022.991590. eCollection 2022.

Hrdina AIH, Kohale IN, Kaushal S, Kelly J, Selin NE, Engelward BP, Kroll JH. The parallel transformations of polycyclic aromatic hydrocarbons in the body and in the atmosphere. Environ. Health Perspect. 2022 Feb;130(2):25004. doi: 10.1289/EHP9984. Epub 2022 Feb 28. PMID: 35225689; PMCID: PMC8884122.

Ngo LP, Kaushal S, Chaim IA, Mazzucato P, Ricciardi C, Samson LD, Nagel ZD, Engelward BP. CometChip analysis of human primary lymphocytes enables quantification of inter-individual differences in the kinetics of repair of oxidative DNA damage. Free Radic Biol Med. 2021 Oct;174:89-99. doi: 10.1016/j.freeradbiomed.2021.07.033. Epub 2021 Jul 26. PMID: 34324980.

Ge J, Ngo LP, Kaushal S, Tay IJ, Thadhani E, Kay JE, Mazzucato P, Chow DN, Fessler JL, Weingeist DM, Sobol RW, Samson LD, Floyd SR, Engelward BP. CometChip enables parallel analysis of multiple DNA repair activities. DNA Repair (Amst). 2021 Oct;106:103176. doi: 10.1016/j.dnarep.2021.103176. Epub 2021 Jul 10. PMID: 34365116

Xiong A, Kaushal S, Tay IJ, Engelward BP, Han J, Preiser PR MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum. STAR Protoc. 2021 Sep 6;2(3):100797. doi: 10.1016/j.xpro.2021.100797. PMID: 34527954

Kelly JM, Ivatt PD, Evans MJ, Kroll JH, Hrdina AIH, Kohale IN, White FM, Engelward BP, Selin NE. Global cancer risk from unregulated polycyclic aromatic hydrocarbons. Geohealth. 2021 Sep 1;5(9):e2021GH000401. doi: 10.1029/2021GH000401. PMID: 34589640

Buick JK, Williams A, Meier MJ, Swartz CD, Recio L, Gagné R, Ferguson SS, Engelward BP, Yauk CL. A modern genotoxicity testing paradigm: integration of the high-throughput CometChip® and the TGx-DDI transcriptomic biomarker in human HepaRG™ cell cultures. Front Public Health. 2021 Aug 18;9:694834. doi: 10.3389/fpubh.2021.694834. PMID: 34485225

Kay JE, Corrigan JJ, Armijo AL, Nazari IS, Kohale IN, Torous DK, Avlasevich SL, Croy RG, Wadduwage DN, Carrasco SE, Dertinger SD, White FM, Essigmann JM, Samson LD, Engelward BP. Excision of mutagenic replication-blocking lesions suppresses cancer but promotes cytotoxicity and lethality in nitrosamine-exposed mice. Cell Rep. 2021 Mar 16;34(11):108864. doi: 10.1016/j.celrep.2021.108864. PMID: 33730582

Engelward BP. Implications of an epidemiological study showing an association between in utero and NDMA exposure and childhood cancer. Mol Mutagen. 2021 Jun;62(5):288-292. doi: 10.1002/em.22434. Epub 2021 Jun 2.PMID: 33963777

Kay JE, Mirabal S, Briley WE, Kimoto T, Poutahidis T, Ragan T, So PT, Wadduwage DN, Erdman SE, Engelward BP. Analysis of mutations in tumor and normal adjacent tissue via fluorescence detection. Environ Mol Mutagen. 2021 Feb;62(2):108-123. doi: 10.1002/em.22419. Epub 2020 Dec 28. PMID: 33314311

Owiti NA, Nagel ZD, Engelward BP. Fluorescence sheds light on DNA damage, DNA repair and mutations. Trends Cancer. 2021 Mar;7(3):240-248. doi: 10.1016/j.trecan.2020.10.006. Epub 2020 Nov 14. PMID: 33203608

Xiong A, Prakash P, Gao X, Chew M, Tay IJJ, Woodrow CJ, Engelward BP, Han J, Preiser PR. K13-medicated reduced susceptibility to artemisinin in plasmodium falciparum is overlaid on a trait of enhanced DNA damage repair. Cell Rep. 2020 Aug 4;32(5):107996. doi: 10.1016/j.celrep.2020.107996. PMID: 32755588

Chao C, Engelward BP. Applications of CometChip for environmental health studies. Chem Res Toxicol. 2020 Jul 20;33(7):1528-1538. doi: 10.1021/acs.chemrestox.9b00393. Epub 2020 Jun 10.
PMID: 32519858

Tay IJ, Park JJH, Price AL, Engelward BP, Floyd SR. HTS-compatible CometChip enables genetic screening for modulators of apoptosis and DNA double-strand break repair. SLAS Discov. 2020 Sep;25(8):906-922. doi: 10.1177/2472555220918367

Ngo LP, Owiti NA, Swartz C, Winters J, Su Y, Ge J, Xiong A, Han J, Recio L, Samson LD, Engelward BP. Sensitive CometChip assay for screening potentially carcinogenic DNA adducts by trapping DNA repair intermediates. Nucleic Acids Res. 2020 Feb 20;48(3):e13.

Wilson MR, Jiang Y, Villalta PW, Stornetta A, Boudreau PD, Carrá A, Brennan CA, Chun E, Ngo L, Samson LD, Engelward BP, Garrett WS, Balbo S, Balskus EP. The human gut bacterial genotoxin colibactin alkylates DNA. Science. 2019 Feb 15;363(6428).

Ngo LP, Chan TK, Ge J, Samson LD, and Engelward BP. Microcolony size distribution assay enables high-throughput cell survival quantitation. Cell Reports. 2019 26:1668-1678.

Kay J, Thadhani E, Samson L, Engelward B. Inflammation-induced DNA damage, mutations and cancer. DNA Repair (Amst). 2019 Nov;83:102673.

Parrish MC, Chaim IA, Nagel ZD, Tannenbaum SR, Samson LD, Engelward BP. Nitric oxide induced S-nitrosation causes base excision repair imbalance. DNA Repair. 2018 68:25-33.

Wadduwage DN, Kay J, Singh VR, Kiraly O, Sukup-Jackson MR, Rajapakse J, Engelward BP, So PTC. Automated fluorescence intensity and gradient analysis enables detection of rare fluorescent mutant cells deep within the tissue of RaDR mice. Sci Rep. 2018 Aug 14;8(1):12108

Sykora P, Witt KL, Revanna P, Smith-Roe SL, Dismukes J, Lloyd DG, Engelward BP, Sobol RW. Next generation high throughput DNA damage detection platform for genotoxic compound screening. Scientific Reports. 2018 Feb 9;8(1):2771.

Townsend TA, Parrish MC, Engelward BP, Manjanatha MG. The development and validation of EpiComet-Chip, a modified high-throughput comet assay for the assessment of DNA methylation status. Environmental and Molecular Mutagenesis. 2017 Aug;58(7):508-521.

Nagel ZD, Engelward BP, Brenner DJ, Begley TJ, Sobol RW, Bielas JH, Stambrook PJ, Wei Q, Hu JJ, Terry MB, Dilworth C, McAllister KA, Reinlib L, Worth L, Shaughnessy DT. Towards precision prevention: Technologies for identifying healthy individuals with high risk of disease. Mutation Research. 2017 Aug;800-802:14-28.

Kimoto T, Kay JE, Li N, Engelward BP. Recombinant cells in the lung increase with age via de novo recombination events and clonal expansion. Environmental and Molecular Mutagenesis. 2017 Apr;58(3):135-145.

Chan TK, Loh XY, Peh HY, Tan WNF, Tan WSD, Li N, Tay IJJ, Wong WSF, Engelward BP. House dust mite-induced asthma causes oxidative damage and DNA double-strand breaks in the lungs. The Journal of Allergy and Clinical Immunology, in press.

Rai P, Parrish M, Tay IJ, Li N, Ackerman S, He F, Kwang J, Chow VT, Engelward BP. (2015) Streptococcus pneumoniae secretes hydrogen peroxide leading to DNA damage and apoptosis in lung cells. Proc. Natl. Acad. Sci. USA. Jun 30;112(26):E3421-30.

Na L, Parrish M, Chan T, Yin L, Rai P, Yamada Y, Kiraly O, Chow VTK, Engelward BP. Influenza infection induces host DNA damage and dynamic DNA damage 1 responses during tissue regeneration. Cell and Molecular Life Sciences. 2015, Aug;72(15):2973-88.

Kiraly O, Gong G, Olipitz W, Muthupalani S, Engelward BP. Inflammation-induced cell proliferation potentiates DNA damage-induced mutations in vivo. PLoS Genet. 2015 Feb 3;11(2):e1004901.

Ge J, Chow DN, Fessler JL, Weingeist DM, Wood DK, Engelward BP. Micropatterned comet assay enables high throughput and sensitive DNA damage quantification.Mutagenesis. 2015 Jan;30(1):11-9.

Ge J, Prasongtanakij S, Wood DK, Weingeist DM, Fessler J, Navasummrit P, Ruchirawat M, and Engelward BP. CometChip: a high-throughput 96-well platform for measuring DNA damage in microarrayed human cells. J Vis Exp. 2014 Oct 18;(92):e50607

Tan KS, Choi H, Jiang X, Yin L, Seet JE, Patzel V, Engelward BP, Chow VT. Micro-RNAs in regenerating lungs: an integrative systems biology analysis of murine influenza pneumonia. BMC Genomics, 2014 15:587.

Kiraly O, Gong G, Roytman MD, Yamada Y, Samson LD, Engelward BP. DNA glycosylase activity and cell proliferation are key factors in modulating homologous recombination in vivo. Carcinogenesis. 2014 Aug 25. pii: bgu177.

Sukup-Jackson MR, Kiraly O, Kay JE, Na L, Rowland EA, Winther KE, Chow DN, Kimoto T, Matsuguchi T, Jonnalagadda VS, Maklakova VI, Singh VR, Wadduwage DN, Rajapakse J, So PT, Collier LS, Engelward BP. Rosa26-GFP direct repeat (RaDR-GFP) mice reveal tissue- and age-dependence of homologous recombination in mammals in vivo. PLoS Genet. 2014 Jun5;10(6):e1004299.

Watson C, Ge J, Cohen J, Pyrgiotakis G, Engelward BP, Demokritou P. High-throughput screening platform for engineered nanoparticle-mediated genotoxicity using CometChip technology. ACS Nano. 2014 Mar 25;8(3):2118-33.

Ge J, Wood DK, Weingeist DM, Prasongtanakig S, Navasumrit P, Ruchirawat M, Engelward BP. Standard fluorescent imaging of live cells is highly genotoxic. Cytometry A. 2013 Jun;83(6):552-60.

Weingeist DM, Ge J, Wood DK, Mutamba JT, Huang Q, Rowland EA, Yaffe MB, Floyd S, and Engelward BP. Single cell microarray enables high throughput evaluation of DNA double strand breaks and DNA repair inhibitors. Cell Cycle. 2013 Mar 15;12(6):907-15.

Li N, Yin L, Thévenin D, Yoshiyuki Y, Limmon G, Chen J, Chow VT, Engelman DM, Engelward BP. Peptide targeting and imaging of damaged lung tissue in influenza-infected mice. Future Microbiol. 2013 Feb;8(2):257-69.

Olipitz W, Wiktor-Brown D, Shuga J, Pang B, McFaline J, Lonkar P, Thomas A, Mutamba JT, Greenberger JS, Samson LD, Dedon PC, Yanch JC, Engelward BP. Integrated molecular analysis indicates undetectable change in DNA damage in mice after continuous irradiation at ~ 400-fold natural background radiation. Environ Health Perspect. 2012 Aug;120(8):1130-6.

Yamada Y, Limmon GV, Zheng D, Li N, Li L, Yin L, Chow VT, Chen J, Engelward BP. Major shifts in the spatio-temporal distribution of lung antioxidant enzymes during influenza pneumonia. PLoS One. 2012;7(2):e31494.

Mutamba JT, Svilar D, Prasongtanakii S, Wang XH, Lin YC, Dedon PC, Sobol RW, Engelward BP. XRCC1 and base excision repair balance in response to nitric oxide. DNA Repair (Amst). 2011 Dec 10;10(12):1282-93.

Wiktor-Brown DM, Sukup-Jackson MR, Fakhraldeen SA, Hendricks CA, Engelward BP. p53 null fluorescent yellow direct repeat (FYDR) mice have normal levels of homologous recombination. DNA Repair (Amst). 2011 Dec 10;10(12):1294-9.

Rugo RE, Mutamba JT, Mohan KN, Yee T, Chaillet JR, Greenberger JS, and Engelward BP. Methyltransferases mediate cell memory of a genotoxic insult. Oncogene. 2011 Feb 10;30(6):751-756.

Wood DK, Weingeist DM, Bhatia SN, Engelward BP. Single cell trapping and DNA damage analysis using microwell arrays. Proc Natl Acad Sci U S A. 2010 Jun 1;107(22):10008-13.

Olipitz W, Hembrador S, Davidson M, Yanch JC, Engelward BP. Development and characterization of a novel variable low dose-rate irradiator for in vivo mouse studies. Health Phys. 2010 May;98(5):727-34.

Wiktor-Brown DM, Kwon HS, Nam YS, So PT, Engelward BP. Integrated one- and two-photon imaging platform reveals clonal expansion as a major driver of mutation load. Proc Natl Acad Sci U S A. 2008 Jul 29;105(30):10314-9.

Wiktor-Brown DM, Olipitz W, Hendricks CA, Rugo RE, Engelward BP. Tissue-specific differences in the accumulation of sequence rearrangements with age. DNA Repair (Amst). 2008 May 3;7(5):694-703.

Helleday T, Lo J, van Gent DC, Engelward BP. DNA double-strand break repair: from mechanistic understanding to cancer treatment. DNA Repair (Amst). 2007 Jul 1;6(7):923-35.

Wiktor-Brown DM, Hendricks CA, Olipitz W, Engelward BP. Age-dependent accumulation of recombinant cells in the mouse pancreas revealed by in situ fluorescence imaging. Proc Natl Acad Sci U S A. 2006 Aug 8;103(32):11862-7.

Nowosielska A, Smith SA, Engelward BP, Marinus MG. Homologous recombination prevents methylation-induced toxicity in Escherichia coli. Nucleic Acids Res. 2006 May 2;34(8):2258-68.

Rugo RE, Almeida KH, Hendricks CA, Jonnalagadda VS, Engelward BP. A single acute exposure to a chemotherapeutic agent induces hyper-recombination in distantly descendant cells and in their neighbors. Oncogene. 2005 Jul 28;24(32):5016-25.

Jonnalagadda VS, Matsuguchi T, Engelward BP. Interstrand crosslink-induced homologous recombination carries an increased risk of deletions and insertions. DNA Repair (Amst). 2005 May 2;4(5):594-605.

Kiziltepe T, Yan A, Dong M, Jonnalagadda VS, Dedon PC, Engelward BP. Delineation of the chemical pathways underlying nitric oxide-induced homologous recombination in mammalian cells. Chem Biol. 2005 Mar;12(3):357-69.